GZnP2 in pcDNA3.1+
(Plasmid
#161739)
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PurposeCytosolic fluorescent reporter for Zn²⁺ (Kd = 352 pM, ex/em = 488 nm/515 nm)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 161739 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1+
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5411
- Total vector size (bp) 6353
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGZnP2
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Alt nameGreen Zn²⁺ Probe 2
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SpeciesSynthetic
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Insert Size (bp)942
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GZnP2 in pcDNA3.1+ was a gift from Yan Qin (Addgene plasmid # 161739 ; http://n2t.net/addgene:161739 ; RRID:Addgene_161739) -
For your References section:
Optical Recording of Zn(2+) Dynamics in the Mitochondrial Matrix and Intermembrane Space with the GZnP2 Sensor. Fudge DH, Black R, Son L, LeJeune K, Qin Y. ACS Chem Biol. 2018 Jul 20;13(7):1897-1905. doi: 10.1021/acschembio.8b00319. Epub 2018 Jun 26. 10.1021/acschembio.8b00319 PubMed 29912548
Map uploaded by the depositor.
