Skip to main content
Addgene

GZnP3 in pcDNA3.1+
(Plasmid #161738)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 161738 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1+
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5411
  • Total vector size (bp) 6353
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GZnP3
  • Alt name
    Green Zn²⁺ Probe 3
  • Species
    Synthetic
  • Insert Size (bp)
    942
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GZnP3 in pcDNA3.1+ was a gift from Yan Qin (Addgene plasmid # 161738 ; http://n2t.net/addgene:161738 ; RRID:Addgene_161738)
  • For your References section:

    Sub-nanomolar sensitive GZnP3 reveals TRPML1-mediated neuronal Zn(2+) signals. Minckley TF, Zhang C, Fudge DH, Dischler AM, LeJeune KD, Xu H, Qin Y. Nat Commun. 2019 Oct 22;10(1):4806. doi: 10.1038/s41467-019-12761-x. 10.1038/s41467-019-12761-x PubMed 31641116