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Addgene

pBabe_Zeo_cOVA_T2A_mStrawberry
(Plasmid #161736)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 161736 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBabe-zeo
  • Backbone manufacturer
    Addgene #1766
  • Total vector size (bp) 6365
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cytoplasmic ovalbumin
  • Alt name
    cOVA
  • Species
    G. gallus (chicken)
  • Tag / Fusion Protein
    • mStrawberry (C terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    cOVA sequence was subcloned from pCI-neo-cOVA (Addgene #25097).

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe_Zeo_cOVA_T2A_mStrawberry was a gift from Alec Kimmelman (Addgene plasmid # 161736 ; http://n2t.net/addgene:161736 ; RRID:Addgene_161736)
  • For your References section:

    Autophagy promotes immune evasion of pancreatic cancer by degrading MHC-I. Yamamoto K, Venida A, Yano J, Biancur DE, Kakiuchi M, Gupta S, Sohn ASW, Mukhopadhyay S, Lin EY, Parker SJ, Banh RS, Paulo JA, Wen KW, Debnath J, Kim GE, Mancias JD, Fearon DT, Perera RM, Kimmelman AC. Nature. 2020 May;581(7806):100-105. doi: 10.1038/s41586-020-2229-5. Epub 2020 Apr 22. 10.1038/s41586-020-2229-5 PubMed 32376951