pBabe_Zeo_cOVA_T2A_mStrawberry
(Plasmid
#161736)
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PurposeExpression of cOVA and mStrawberry
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 161736 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBabe-zeo
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Backbone manufacturerAddgene #1766
- Total vector size (bp) 6365
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Vector typeMammalian Expression, Retroviral
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecytoplasmic ovalbumin
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Alt namecOVA
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SpeciesG. gallus (chicken)
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Tag
/ Fusion Protein
- mStrawberry (C terminal on insert)
Resource Information
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A portion of this plasmid was derived from a plasmid made bycOVA sequence was subcloned from pCI-neo-cOVA (Addgene #25097).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBabe_Zeo_cOVA_T2A_mStrawberry was a gift from Alec Kimmelman (Addgene plasmid # 161736 ; http://n2t.net/addgene:161736 ; RRID:Addgene_161736) -
For your References section:
Autophagy promotes immune evasion of pancreatic cancer by degrading MHC-I. Yamamoto K, Venida A, Yano J, Biancur DE, Kakiuchi M, Gupta S, Sohn ASW, Mukhopadhyay S, Lin EY, Parker SJ, Banh RS, Paulo JA, Wen KW, Debnath J, Kim GE, Mancias JD, Fearon DT, Perera RM, Kimmelman AC. Nature. 2020 May;581(7806):100-105. doi: 10.1038/s41586-020-2229-5. Epub 2020 Apr 22. 10.1038/s41586-020-2229-5 PubMed 32376951