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PurposeDoxycycline-inducible expression of mStrawberry
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 161734 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepINDUCER20
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Backbone manufacturerAddgene #44012
- Total vector size (bp) 10859
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Vector typeMammalian Expression, Lentiviral ; destinatioin vector for gateway cloning
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemStrawberry
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer attB1
- 3′ sequencing primer attB2 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pINDUCER20_mStrawberry was a gift from Alec Kimmelman (Addgene plasmid # 161734 ; http://n2t.net/addgene:161734 ; RRID:Addgene_161734) -
For your References section:
Autophagy promotes immune evasion of pancreatic cancer by degrading MHC-I. Yamamoto K, Venida A, Yano J, Biancur DE, Kakiuchi M, Gupta S, Sohn ASW, Mukhopadhyay S, Lin EY, Parker SJ, Banh RS, Paulo JA, Wen KW, Debnath J, Kim GE, Mancias JD, Fearon DT, Perera RM, Kimmelman AC. Nature. 2020 May;581(7806):100-105. doi: 10.1038/s41586-020-2229-5. Epub 2020 Apr 22. 10.1038/s41586-020-2229-5 PubMed 32376951