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pSLIK_Hyg_mTurquoise2_Atg4BC74A
(Plasmid #161733)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 161733 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSLIK-hygro
  • Backbone manufacturer
    Addgene #25737
  • Backbone size w/o insert (bp) 12262
  • Total vector size (bp) 14694
  • Vector type
    Mammalian Expression, Lentiviral ; destinatioin vector for gateway cloning
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    After plasmid purification, please make sure your plasmid contains 7 repeats of TetO sequence as this region can occasionally be lost by recombination.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Atg4B(C74A) (Atg4b Mouse)
  • Alt name
    Atg4B
  • Species
    M. musculus (mouse)
  • Mutation
    220-222 TGC (Cys) is changed to GCA (Ala)
  • GenBank ID
    NM_174874
  • Entrez Gene
    Atg4b (a.k.a. 2510009N07Rik, Apg4b, Atg4bl, Autl1, MmAPG4B)
  • Tag / Fusion Protein
    • mTurquoise2 (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSLIK_Hyg_mTurquoise2_Atg4BC74A was a gift from Alec Kimmelman (Addgene plasmid # 161733 ; http://n2t.net/addgene:161733 ; RRID:Addgene_161733)

  • For your References section:

    Autophagy promotes immune evasion of pancreatic cancer by degrading MHC-I. Yamamoto K, Venida A, Yano J, Biancur DE, Kakiuchi M, Gupta S, Sohn ASW, Mukhopadhyay S, Lin EY, Parker SJ, Banh RS, Paulo JA, Wen KW, Debnath J, Kim GE, Mancias JD, Fearon DT, Perera RM, Kimmelman AC. Nature. 2020 May;581(7806):100-105. doi: 10.1038/s41586-020-2229-5. Epub 2020 Apr 22. 10.1038/s41586-020-2229-5 PubMed 32376951
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