pNDL-60
(Plasmid
#161720)
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PurposeIn vitro transcription construct for production of cytosolic acvr2b-CIB1N mRNA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 161720 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2
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Vector typeIn vitro transcription
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecytosolic acvr2b-CIB1N
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SpeciesD. rerio (zebrafish)
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Entrez Geneacvr2b (a.k.a. actr2b, actrIIb, wu:fj97d11)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.04.11.588875 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNDL-60 was a gift from Adam Cohen (Addgene plasmid # 161720 ; http://n2t.net/addgene:161720 ; RRID:Addgene_161720) -
For your References section:
Optogenetic control of Nodal signaling patterns. McNamara HM, Jia BZ, Guyer A, Parot VJ, Dobbs C, Schier AF, Cohen AE, Lord ND. bioRxiv [Preprint]. 2024 Apr 12:2024.04.11.588875. doi: 10.1101/2024.04.11.588875. 10.1101/2024.04.11.588875 PubMed 38645239