pMH-EBFP
(Plasmid
#161714)
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Purpose(Empty Backbone) Gateway-compatible destination vector for mammalian expression of N-terminal EBFP protein under CMV promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 161714 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMH-EBFP
- Backbone size (bp) 7636
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Vector typeMammalian Expression
- Promoter cmv
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Selectable markersPuromycin
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Tag
/ Fusion Protein
- EBFP (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMH-EBFP was a gift from Michael Huen (Addgene plasmid # 161714 ; http://n2t.net/addgene:161714 ; RRID:Addgene_161714)
