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Addgene

p alpha-actinin:GAL4/UAS:TdTomato
(Plasmid #161712)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 161712 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBlueScriptSK
  • Vector type
    Mammalian Expression ; Xenopus laevis expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TdTomato, GAL4
  • Species
    S. cerevisiae (budding yeast), Synthetic; Discosoma sp.
  • Insert Size (bp)
    3100
  • Promoter actin-GAL4, UAS
  • Tag / Fusion Protein
    • TdTomato

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (destroyed during cloning)
  • 3′ cloning site None (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Additional references:
-Koster et. al, Dev Biol. 2001, 233(2):329-46. doi.org/10.1006/dbio.2001.0242
-Bestman, J.E., and Cline, H.T. PNAS. 2008, 105: 20494-20499. doi.org/10.1073/pnas.0806296105
-Gabmrill et. al, J Neurophysiol. 2018, 119: 1947–1961. doi.org/10.1152/jn.00051.2018

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p alpha-actinin:GAL4/UAS:TdTomato was a gift from Hollis Cline (Addgene plasmid # 161712 ; http://n2t.net/addgene:161712 ; RRID:Addgene_161712)

  • For your References section:

    Optic flow instructs retinotopic map formation through a spatial to temporal to spatial transformation of visual information. Hiramoto M, Cline HT. Proc Natl Acad Sci U S A. 2014 Nov 25;111(47):E5105-13. doi: 10.1073/pnas.1416953111. Epub 2014 Nov 10. 10.1073/pnas.1416953111 PubMed 25385606
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