pENTR-GFI1
(Plasmid #16168)

• Depositing Lab: Huda Zoghbi
• Publication: Lim et al Cell. 2006 May 19. 125(4):801-14.

Backbone

• Vector backbone: pDONR223
• Backbone size w/o insert (bp): 5005
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Growth factor independent 1
• Alt name: GFI1
• Species: H. sapiens (human)
• Insert Size (bp): 1266
• Entrez Gene: GFI1 (a.k.a. GFI-1, GFI1A, SCN2, ZNF163)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: attP1 (destroyed during cloning)
• 3′ cloning site: attP2 (destroyed during cloning)
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Full-length human cDNAs were amplified using PCR primers and cloned into the pDONR223 "Donor" vector using the Gateway system as described (Rual et al., 2004), resulting in "Entry" clones. Reverse primers do not include stop codons. Forward primer: 5'-GGGGACAACTTTGTACAAAAAAGTTGGC ATGCCGCGCTCATTTCTCGT-3'. Reverse primer: 5'-GGGGACAACTTTGTACAAGAAAGTTGG TTTGAGCCCATGCTGCGTCT-3'.

There is a P->L mutation in the second amino acid. The depositing lab is aware of this and do not believe it affects the function of the protein.