IDG_GRID1_OE_1
(Plasmid
#161661)
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PurposeOverexpresses GRID1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 161661 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLX304
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGRID1
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SpeciesH. sapiens (human)
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Entrez GeneGRID1 (a.k.a. KIAA1220)
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Tag
/ Fusion Protein
- V5 (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note this plasmid contains two GRID1 inserts and additional backbone elements. Depositor states this affects plasmid function but the plasmid can be used for cloning purposes.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
IDG_GRID1_OE_1 was a gift from Mike McManus (Addgene plasmid # 161661 ; http://n2t.net/addgene:161661 ; RRID:Addgene_161661)