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pAAV-hSyn-miRFP-6xFLAG
(Plasmid #161614)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 161614 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-hSyn
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4496
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    miRFP-6xFLAG
  • Alt name
    3xFLAG-miRFP-3xFLAG
  • Alt name
    smiRFP_FLAG
  • Species
    Synthetic
  • Insert Size (bp)
    1131
  • Mutation
    N/A
  • Promoter Human synapsin 1 promoter

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The fluorescence of miRFP will be quenched after formaldehyde fixation. The pAAV-hSyn backbone contains WHP Posttranscriptional Response Element (WPRE) at 3'-UTR.

5' cloning site: EcoRI (not destroyed), 3' cloning site: HindIII (not destroyed).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hSyn-miRFP-6xFLAG was a gift from Edward Boyden (Addgene plasmid # 161614 ; http://n2t.net/addgene:161614 ; RRID:Addgene_161614)

  • For your References section:

    Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling Network Dynamics. Linghu C, Johnson SL, Valdes PA, Shemesh OA, Park WM, Park D, Piatkevich KD, Wassie AT, Liu Y, An B, Barnes SA, Celiker OT, Yao CC, Yu CJ, Wang R, Adamala KP, Bear MF, Keating AE, Boyden ES. Cell. 2020 Nov 17. pii: S0092-8674(20)31399-4. doi: 10.1016/j.cell.2020.10.035. 10.1016/j.cell.2020.10.035 PubMed 33232692
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