pMSP1E1D1
(Plasmid #161605)

• Purpose: Bacterial expression of "extended" membrane scaffold protein (MSP1E1D1) for Nanodisc formation
• Depositing Lab: Stephen Sligar
• Publication: Ritchie et al Methods Enzymol. 2009 . 464():211-31.

Backbone

• Vector backbone: pET 28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5300
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: for protein expression use strain with internal source of T7 polymerase e.g. BL21(DE3)
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MSP1E1D1
• Alt name: membrane scaffold protein E1D1
• Alt name: APOA1
• Species: Synthetic; synthetic gene
• Insert Size (bp): 715
• Mutation: "extended" MSP1D1; contains repeat of helix 4
• Promoter: T7
• Tag / Fusion Protein:
  • 7-His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nco 1 (not destroyed)
• 3′ cloning site: Hind III (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry