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Addgene

LTRi Cre
(Plasmid #16159)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16159 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    LTRi
  • Backbone size w/o insert (bp) 8000
  • Vector type
    Mammalian Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cre recombinase
  • Alt name
    cre
  • Species
    Bacteriophage P1
  • Insert Size (bp)
    2000
  • Entrez Gene
    cre (a.k.a. P1_gp003)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

We engineered a tunable genetic switch called LTRi, which stands for Lac-Tet-RNAi. Gene expression is turned on by adding an inducer, isopropyl-β-thiogalactopyranoside (IPTG). LTRi-Cre allows scientists to tune the level of Cre. See article for details.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LTRi Cre was a gift from James Collins (Addgene plasmid # 16159)

  • For your References section:

    A tunable genetic switch based on RNAi and repressor proteins for regulating gene expression in mammalian cells. Deans TL, Cantor CR, Collins JJ. Cell. 2007 Jul 27. 130(2):363-72. 10.1016/j.cell.2007.05.045 PubMed 17662949
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