pPuro3.1(+)_Strep-Lrrk2(delKinase)-Myc
(Plasmid #161586)

• Purpose: expression of Myc-tagged mouse Lrrk2 without kinase domain
• Depositing Lab: Maik Hintze
• Publication: Lrrk2 expression plasmids (unpublished)

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone size w/o insert (bp): 4902
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Growth instructions: Depositor recommends growth at 30°C for 24 hrs.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lrrk2
• Species: M. musculus (mouse)
• Insert Size (bp): 5660
• Mutation: deletion: AA1871-2540
• Entrez Gene: Lrrk2 (a.k.a. 4921513O20Rik, 9330188B09Rik, D630001M17Rik, Gm927, cI-46)
• Promoter: CMV
• Tags / Fusion Proteins:
  • StrepII (N terminal on insert)
  • Myc tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: ApaI (unknown if destroyed)
• 5′ sequencing primer: GGC TAA CTA GAG AAC CCA CTG
• 3′ sequencing primer: GGC AAC TAG AAG GCA CAG TC

Resource Information

• Supplemental Documents:
  • pPuro3.1(+)_Strep-Lrrk2delKinase-Myc.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Neo cassette from pcDNA3.1(+) replaced with Puro cassette from pPUR (Clontech/TaKaRa).