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pET20b-delta133HtrA2 G399S
(Plasmid #16158)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16158 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET20b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3584
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    When transformed and plated on LB/agar/Amp plates, this plasmid will normally only produce colonies if the plates are incubated at room temp as opposed to 37oC. Following this, the bacteria when inoculated in liquid media will grow well at 37oC.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HtrA2
  • Alt name
    Omi
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    978
  • Mutation
    G399S
  • GenBank ID
    NM_013247
  • Entrez Gene
    HTRA2 (a.k.a. MGCA8, OMI, PARK13, PRSS25)
  • Promoter T7
  • Tag / Fusion Protein
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This construct was used in experiments described in figure 4

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET20b-delta133HtrA2 G399S was a gift from L. Miguel Martins (Addgene plasmid # 16158 ; http://n2t.net/addgene:16158 ; RRID:Addgene_16158)

  • For your References section:

    Loss of function mutations in the gene encoding Omi/HtrA2 in Parkinson's disease. Strauss KM, Martins LM, Plun-Favreau H, Marx FP, Kautzmann S, Berg D, Gasser T, Wszolek Z, Muller T, Bornemann A, Wolburg H, Downward J, Riess O, Schulz JB, Kruger R. Hum Mol Genet. 2005 Aug 1. 14(15):2099-111. 10.1093/hmg/ddi215 PubMed 15961413
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