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pET20b-delta133HtrA2 S142D
(Plasmid #16154)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16154 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET20b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3584
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    When transformed and plated on LB/agar/Amp plates, this plasmid will normally only produce colonies if the plates are incubated at room temp as opposed to 37oC. Following this, the bacteria when inoculated in liquid media will grow well at 37oC.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HtrA2
  • Alt name
    Omi
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    978
  • Mutation
    S142D
  • GenBank ID
    NM_013247
  • Entrez Gene
    HTRA2 (a.k.a. MGCA8, OMI, PARK13, PRSS25)
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This construct was used in experiments described in figure 4

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET20b-delta133HtrA2 S142D was a gift from L. Miguel Martins (Addgene plasmid # 16154 ; http://n2t.net/addgene:16154 ; RRID:Addgene_16154)

  • For your References section:

    The mitochondrial protease HtrA2 is regulated by Parkinson's disease-associated kinase PINK1. Plun-Favreau H, Klupsch K, Moisoi N, Gandhi S, Kjaer S, Frith D, Harvey K, Deas E, Harvey RJ, McDonald N, Wood NW, Martins LM, Downward J. Nat Cell Biol. 2007 Sep 30. ():. 10.1038/ncb1644 PubMed 17906618
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