pYPQ262-ABE8e
(Plasmid
#161523)
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PurposeGateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e mediated A-G base editing
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 161523 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepYPQ166
- Total vector size (bp) 8414
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Vector typePlant Expression, CRISPR ; Gateway compatible ABE8e-zSpCas9(D10A) entry clone
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameABE8e-zSpCas9(D10A)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYPQ262-ABE8e was a gift from Yiping Qi (Addgene plasmid # 161523 ; http://n2t.net/addgene:161523 ; RRID:Addgene_161523) -
For your References section:
PAM-less plant genome editing using a CRISPR-SpRY toolbox. Ren Q, Sretenovic S, Liu S, Tang X, Huang L, He Y, Liu L, Guo Y, Zhong Z, Liu G, Cheng Y, Zheng X, Pan C, Yin D, Zhang Y, Li W, Qi L, Li C, Qi Y, Zhang Y. Nat Plants. 2021 Jan 4. pii: 10.1038/s41477-020-00827-4. doi: 10.1038/s41477-020-00827-4. 10.1038/s41477-020-00827-4 PubMed 33398158