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Addgene

pYPQ166-SpRY
(Plasmid #161520)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 161520 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYPQ166
  • Total vector size (bp) 7775
  • Vector type
    Plant Expression, CRISPR ; Gateway compatible zSpRYCas9 entry clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    zSpRYCas9
  • Alt name
    3xFLAG-NLS-zSpRYCas9-NLS
  • Tags / Fusion Proteins
    • 3X FLAG, NLS (N terminal on insert)
    • NLS (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYPQ166-SpRY was a gift from Yiping Qi (Addgene plasmid # 161520 ; http://n2t.net/addgene:161520 ; RRID:Addgene_161520)

  • For your References section:

    PAM-less plant genome editing using a CRISPR-SpRY toolbox. Ren Q, Sretenovic S, Liu S, Tang X, Huang L, He Y, Liu L, Guo Y, Zhong Z, Liu G, Cheng Y, Zheng X, Pan C, Yin D, Zhang Y, Li W, Qi L, Li C, Qi Y, Zhang Y. Nat Plants. 2021 Jan 4. pii: 10.1038/s41477-020-00827-4. doi: 10.1038/s41477-020-00827-4. 10.1038/s41477-020-00827-4 PubMed 33398158
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