GPX8 rescue PLAK1
(Plasmid
#161517)
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PurposeRescues the expression of GPX8 in GPX8 plenti-CRISPR KO cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 161517 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePLAK1
- Total vector size (bp) 8284
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Vector typeLentiviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameglutathione peroxidase 8
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Alt nameGPX8
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SpeciesH. sapiens (human)
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GenBank ID
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Entrez GeneGPX8 (a.k.a. EPLA847, GPx-8, GSHPx-8, UNQ847)
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Tag
/ Fusion Protein
- 3X Flag
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GPX8 rescue PLAK1 was a gift from Yoav Shaul (Addgene plasmid # 161517 ; http://n2t.net/addgene:161517 ; RRID:Addgene_161517) -
For your References section:
The glutathione peroxidase 8 (GPX8)/IL-6/STAT3 axis is essential in maintaining an aggressive breast cancer phenotype. Khatib A, Solaimuthu B, Ben Yosef M, Abu Rmaileh A, Tanna M, Oren G, Schlesinger Frisch M, Axelrod JH, Lichtenstein M, Shaul YD. Proc Natl Acad Sci U S A. 2020 Sep 1;117(35):21420-21431. doi: 10.1073/pnas.2010275117. Epub 2020 Aug 18. 10.1073/pnas.2010275117 PubMed 32817494