pCFJ1149 - intron 3 smu-2 PATCs
(Plasmid #161516)

• Purpose: PATC-rich intron number three from smu-2 to stimulate germline expression in C. elegans
• Depositing Lab: Christian Froekjaer-Jensen
• Publication: Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi: 10.1038/s41467-020-19898-0.

Backbone

• Vector backbone: pDONR221
• Backbone manufacturer: ThermoFisher
• Backbone size w/o insert (bp): 2558
• Total vector size (bp): 3774
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Intron 3 from smu-2
• Species: C. elegans (nematode)
• Insert Size (bp): 1176

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • pCFJ1149.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Intron number 3 from the gene smu-2. This intron is enriched for PATCs can can partially prevent transgene silencing in the C. elegans germline.