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PurposeNeuronal expression of histamine-sensitive chloride channel for negative selection
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 161515 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDEST R4-R3
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Backbone manufacturerThermoFischer
- Backbone size w/o insert (bp) 4107
- Total vector size (bp) 7663
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepEXP[Psnt-1 | histamine | rpl-3 UTR]
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SpeciesC. elegans (nematode)
- Promoter Psnt-1
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Synthetic Genome Biology lab @ KAUST www.wormbuilder.org
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSEM238 was a gift from Christian Froekjaer-Jensen (Addgene plasmid # 161515 ; http://n2t.net/addgene:161515 ; RRID:Addgene_161515) -
For your References section:
A histamine-gated channel is an efficient negative selection marker for C. elegans transgenesis. El Mouridi S, AlHarbi S, Frokjaer-Jensen C. MicroPubl Biol. 2021 Jan 8;2021. doi: 10.17912/micropub.biology.000349. 10.17912/micropub.biology.000349 PubMed 33437931