pLenti-PE2-BSD
(Plasmid #161514)

• Purpose: Expression of PE2 in mammalian cells. Can be used to generate stable cell lines by Blasticidin selection
• Depositing Lab: Hyongbum Kim
• Publication: Kim et al Nat Biotechnol. 2020 Sep 21. pii: 10.1038/s41587-020-0677-y. doi: 10.1038/s41587-020-0677-y.

Backbone

• Vector backbone: lentiCas9-Blast
• Backbone manufacturer: Feng Zhang
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PE2-P2A-BSD
• Alt name: Prime editor 2-P2A-Blast
• Alt name: Cas9(H840A)-M-MLV reversetranscriptase (D200N, T306K, W313F, T330P, L603W)-P2A-BSD
• Species: Synthetic
• Insert Size (bp): 6816
• Promoter: EF-1α core promoter
• Tags / Fusion Proteins:
  • SV40 NLS (N terminal on insert)
  • SV40 NLS (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: EFS_forward (5'-GGTCGGCAATTGATCCGGTG-3')
• 3′ sequencing primer: WPRE_reverse (5'-GGTTGCGTCAGCAAACACAG-3')

Resource Information

• Supplemental Documents:
  • pLenti-PE2-BSD.gb
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry