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Addgene

pLenti-PE2-BSD
(Plasmid #161514)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 161514 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lentiCas9-Blast
  • Backbone manufacturer
    Feng Zhang
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PE2-P2A-BSD
  • Alt name
    Prime editor 2-P2A-Blast
  • Alt name
    Cas9(H840A)-M-MLV reversetranscriptase (D200N, T306K, W313F, T330P, L603W)-P2A-BSD
  • Species
    Synthetic
  • Insert Size (bp)
    6816
  • Promoter EF-1α core promoter
  • Tags / Fusion Proteins
    • SV40 NLS (N terminal on insert)
    • SV40 NLS (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer EFS_forward (5'-GGTCGGCAATTGATCCGGTG-3')
  • 3′ sequencing primer WPRE_reverse (5'-GGTTGCGTCAGCAAACACAG-3')
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-PE2-BSD was a gift from Hyongbum Kim (Addgene plasmid # 161514 ; http://n2t.net/addgene:161514 ; RRID:Addgene_161514)

  • For your References section:

    Predicting the efficiency of prime editing guide RNAs in human cells. Kim HK, Yu G, Park J, Min S, Lee S, Yoon S, Kim HH. Nat Biotechnol. 2020 Sep 21. pii: 10.1038/s41587-020-0677-y. doi: 10.1038/s41587-020-0677-y. 10.1038/s41587-020-0677-y PubMed 32958957
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