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Addgene

pBScMXT + mSlo1 MBr5/2
(Plasmid #16150)

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Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16150 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBScMXT
  • Backbone size w/o insert (bp) 3200
  • Vector type
    Xenopus Oocyte expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Mus musculus potassium large conductance calcium-activated channel
  • Alt name
    mSlo1
  • Alt name
    GI:347143
  • Species
    Mouse brain
  • Insert Size (bp)
    2800
  • Mutation
    truncated brain mSlo1
  • GenBank ID
    L16912
  • Entrez Gene
    Kcnma1 (a.k.a. 5730414M22Rik, BKCA alpha, BKCa, KCa1.1, MaxiK, Slo, Slo1, k(VCA)alpha, mSlo, mSlo1, slo-alpha)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBScMXT + mSlo1 MBr5/2 was a gift from Larry Salkoff (Addgene plasmid # 16150)

  • For your References section:

    mSlo, a complex mouse gene encoding "maxi" calcium-activated potassium channels. Butler A, Tsunoda S, McCobb DP, Wei A, Salkoff L. Science. 1993 Jul 9. 261(5118):221-4. 10.1126/science.7687074 PubMed 7687074
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