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Addgene

pOX + JShal
(Plasmid #16147)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16147 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOX
  • Backbone size w/o insert (bp) 3200
  • Vector type
    Xenopus Oocyte expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Polyorchis penicillatus potassium channel alpha subunit
  • Alt name
    Shal-alpha-1
  • Species
    jelly fish
  • Insert Size (bp)
    1400
  • Mutation
    N/A
  • GenBank ID
    U78642
  • Entrez Gene
    Shal (a.k.a. Dmel_CG9262, CG9262, DNK[[v]]4, Dmel\CG9262, K[[v]]4, Kv4, Kv4/Shal, Sh-l, ShaI, Shl, dShal, shaI, shal)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Xba1 (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOX + JShal was a gift from Larry Salkoff (Addgene plasmid # 16147 ; http://n2t.net/addgene:16147 ; RRID:Addgene_16147)

  • For your References section:

    A novel subunit for shal K+ channels radically alters activation and inactivation. Jegla T, Salkoff L. J Neurosci. 1997 Jan 1. 17(1):32-44. PubMed 8987734
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