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Addgene

pBSc + MShab
(Plasmid #16146)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16146 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBSc
  • Backbone size w/o insert (bp) 3200
  • Vector type
    Xenopus Oocyte expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Mus musculus potassium voltage gated channel
  • Alt name
    GI:200975
  • Alt name
    M64228
  • Species
    M. musculus (mouse), D. melanogaster (fly)
  • Insert Size (bp)
    3400
  • Mutation
    N/A
  • GenBank ID
    NM_008420
  • Entrez Gene
    Kcnb1 (a.k.a. Kcr1-1, Kv2.1, Shab)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sac11 (not destroyed)
  • 3′ cloning site Hind111 (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBSc + MShab was a gift from Larry Salkoff (Addgene plasmid # 16146 ; http://n2t.net/addgene:16146 ; RRID:Addgene_16146)

  • For your References section:

    A mouse brain homolog of the Drosophila Shab K+ channel with conserved delayed-rectifier properties. Pak MD, Covarrubias M, Ratcliffe A, Salkoff L. J Neurosci. 1991 Mar . 11(3):869-80. PubMed 2002364

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