pBSc polyA + Shal2
(Plasmid
#16144)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16144 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBSc
- Backbone size w/o insert (bp) 3200
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Vector typeXenopus Oocyte expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameShal
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Alt nameCG9262-RA
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Alt nameGI:17737641
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Alt nameNP_524159
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SpeciesD. melanogaster (fly)
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Insert Size (bp)2470
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MutationN/A
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GenBank IDNM_079435
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Entrez GeneShal (a.k.a. Dmel_CG9262, CG9262, DNK[[v]]4, Dmel\CG9262, K[[v]]4, Kv4, Kv4/Shal, Sh-l, ShaI, Shl, dShal, shaI, shal)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Hind111 (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBSc polyA + Shal2 was a gift from Larry Salkoff (Addgene plasmid # 16144 ; http://n2t.net/addgene:16144 ; RRID:Addgene_16144) -
For your References section:
A family of putative potassium channel genes in Drosophila. Butler A, Wei AG, Baker K, Salkoff L. Science. 1989 Feb 17. 243(4893):943-7. 10.1126/science.2493160 PubMed 2493160
Map uploaded by the depositor.