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Addgene

pDONR221-TMEM104_STOP
(Plasmid #161428)

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Item Catalog # Description Quantity Price (USD)
Plasmid 161428 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pDONR221
  • Backbone manufacturer
    ThermoFisher Scientific
  • Backbone size w/o insert (bp) 2550
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TMEM104
  • Alt name
    FLJ20255, FLJ00021
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1491
  • Entrez Gene
    TMEM104 (a.k.a. FLJ00021, FLJ20255)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid contains a STOP codon at the end of the codon-optimized ORF. For the version of this plasmid that does not contain a STOP codon, please see Addgene plasmid 132262 For more information on the full Resolute plasmid collection, please see www.addgene.org/depositor-collections/re-solute/. Please provide your feedback on this plasmid to the RESOLUTE consortium by filling out their RESOLUTE repository feedback form: https://forms.office.com/Pages/ResponsePage.aspx?id=0e05yklzmkS7rjFGQL4N7z4feCLQvEJAmVcOCM_u885UN1JJRko0Ukg4TVQwNTZLOUxPQVJWT1NHUCQlQCN0PWcu

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR221-TMEM104_STOP was a gift from RESOLUTE Consortium & Giulio Superti-Furga (Addgene plasmid # 161428 ; http://n2t.net/addgene:161428 ; RRID:Addgene_161428)

  • For your References section:

    The RESOLUTE consortium: unlocking SLC transporters for drug discovery. Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov V, Wolf G, Crawford M, Everley R, ... Steppan CM. Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 10.1038/d41573-020-00056-6. 10.1038/d41573-020-00056-6 PubMed 32265506
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