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Addgene

pBSc + Shal
(Plasmid #16142)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16142 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBSc
  • Backbone size w/o insert (bp) 3200
  • Vector type
    Xenopus Oocyte expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Voltage-gated K+ channels
  • Alt name
    Y73B6BL.19
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    2260
  • Mutation
    N/A
  • GenBank ID
    NM_068574
  • Entrez Gene
    Y73B6BL.19

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBSc + Shal was a gift from Larry Salkoff (Addgene plasmid # 16142 ; http://n2t.net/addgene:16142 ; RRID:Addgene_16142)

  • For your References section:

    Mutant analysis of the Shal (Kv4) voltage-gated fast transient K+ channel in Caenorhabditis elegans. Fawcett GL, Santi CM, Butler A, Harris T, Covarrubias M, Salkoff L. J Biol Chem. 2006 Oct 13. 281(41):30725-35. 10.1074/jbc.M605814200 PubMed 16899454
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