pOX + Shaker1d
(Plasmid
#16141)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16141 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepOX
- Backbone size w/o insert (bp) 3200
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Vector typeXenopus Oocyte expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameVoltage-gated K+ channels
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Alt nameZK1321.2d
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SpeciesC. elegans (nematode)
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Insert Size (bp)1806
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MutationN/A
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GenBank IDNM_182136
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Entrez GeneZK1321.2
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Nhe1 (destroyed during cloning)
- 3′ cloning site Xho1 (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOX + Shaker1d was a gift from Larry Salkoff (Addgene plasmid # 16141 ; http://n2t.net/addgene:16141 ; RRID:Addgene_16141) -
For your References section:
Mutant analysis of the Shal (Kv4) voltage-gated fast transient K+ channel in Caenorhabditis elegans. Fawcett GL, Santi CM, Butler A, Harris T, Covarrubias M, Salkoff L. J Biol Chem. 2006 Oct 13. 281(41):30725-35. 10.1074/jbc.M605814200 PubMed 16899454
Map uploaded by the depositor.