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Addgene

pOX + Shaker1a
(Plasmid #16139)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16139 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOX
  • Backbone size w/o insert (bp) 3200
  • Vector type
    Xenopus Oocyte expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Voltage-gated K+ channels
  • Alt name
    ZK1321.2a
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    1782
  • Mutation
    N/A
  • GenBank ID
    NM_063703
  • Entrez Gene
    ZK1321.2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (destroyed during cloning)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOX + Shaker1a was a gift from Larry Salkoff (Addgene plasmid # 16139 ; http://n2t.net/addgene:16139 ; RRID:Addgene_16139)

  • For your References section:

    Mutant analysis of the Shal (Kv4) voltage-gated fast transient K+ channel in Caenorhabditis elegans. Fawcett GL, Santi CM, Butler A, Harris T, Covarrubias M, Salkoff L. J Biol Chem. 2006 Oct 13. 281(41):30725-35. 10.1074/jbc.M605814200 PubMed 16899454
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