pOX + kqt3
(Plasmid #16138)

• Depositing Lab: Larry Salkoff
• Publication: Wei et al J Biol Chem. 2005 Jun 3. 280(22):21337-45.

Backbone

• Vector backbone: pOX
• Backbone size w/o insert (bp): 3200
• Vector type: Xenopus Oocyte expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Caenorhabditis elegans potassium channel
• Alt name: Y54G9A.3
• Species: C. elegans (nematode)
• Insert Size (bp): 1900
• Mutation: lacks aa1-16 compared to GenBank reference AY572975
• GenBank ID:
• Entrez Gene: kqt-3 (a.k.a. CELE_Y54G9A.3)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sal1 (not destroyed)
• 3′ cloning site: Xho1 (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results indicate the kqt3 insert in this plasmid is lacking the first 16 amino acids when compared to GenBank accession number AY572975 and begins with the sequence MTYRAMINCLERP... According to the depositing laboratory, this is the exact constructed used in the associated publication listed below and it functions as described in the publication.