pOX + kqt2
(Plasmid #16137)

• Depositing Lab: Larry Salkoff
• Publication: Wei et al J Biol Chem. 2005 Jun 3. 280(22):21337-45.

Backbone

• Vector backbone: pOX
• Backbone size w/o insert (bp): 3200
• Vector type: Xenopus Oocyte expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Caenorhabditis elegans potassium channel
• Alt name: M60.5
• Species: C. elegans (nematode)
• Insert Size (bp): 2200
• Mutation: Deletion of aa1-38 compared to GenBank NP_509392.3
• GenBank ID: NM_076991
• Entrez Gene: kqt-2 (a.k.a. CELE_M60.5)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Cla1 (not destroyed)
• 3′ cloning site: Xho1 (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results indicate the kqt2 insert in this plasmid is lacking the first 39 amino acids when compared to GenBank accession number NP_509392.3. This plasmid is the exact construct used and described in the associated publication.