pOX + kqt1
(Plasmid
#16136)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16136 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepOX
- Backbone size w/o insert (bp) 3200
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Vector typeXenopus Oocyte expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCaenorhabditis elegans potassium channel
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Alt nameC25B8.1b
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SpeciesC. elegans (nematode)
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Insert Size (bp)2400
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MutationN/A
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GenBank IDNM_171710
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Entrez Genekqt-1 (a.k.a. CELE_C25B8.1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Nhe1 (not destroyed)
- 3′ cloning site Xho1 (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOX + kqt1 was a gift from Larry Salkoff (Addgene plasmid # 16136 ; http://n2t.net/addgene:16136 ; RRID:Addgene_16136) -
For your References section:
KCNQ-like potassium channels in Caenorhabditis elegans. Conserved properties and modulation. Wei AD, Butler A, Salkoff L. J Biol Chem. 2005 Jun 3. 280(22):21337-45. 10.1074/jbc.M502734200 PubMed 15797864
Map uploaded by the depositor.