pBAD/myc-his A Rv0712 (FGE)
(Plasmid #16132)

• Purpose: Bacterial expression of Mycobacterium tuberculosis FGE
• Depositing Lab: Carolyn Bertozzi
• Publication: Carrico et al Nat Chem Biol. 2007 Jun . 3(6):321-2.

Backbone

• Vector backbone: pBAD/myc-his A
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4081
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Formylglycine-generating enzyme
• Alt name: m tuberculosis FGE
• Alt name: mtb FGE
• Species: Mycobacterium tuberculosis
• Insert Size (bp): 903
• GenBank ID: NP_215226

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (destroyed during cloning)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pBAD-F

Resource Information

• Addgene Notes:
  • Full sequence from requesting scientist
• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The gene encoding Mycobacterium tuberculosis FGE (Rv0712, encoding residues 2-299, GenBank accession NP_215226) was amplified from M. tuberculosis H37Rv genomic DNA and ligated into pBAD/myc-his A between the NcoI and XhoI restriction sites. The FGE gene was placed in frame with the start codon using QuikChange mutagenesis.
Please note that this information page previously listed the NcoI cloning site as not destroyed. The site was destroyed during cloning, however, and the page was updated on 6/16/08.