pcDNAOX-twk46
(Plasmid
#16129)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16129 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNAOX
- Backbone size w/o insert (bp) 5700
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Vector typeMammalian expression & Oocyte expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameVoltage-gated K+ channel
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Alt nameY39B6A.19
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SpeciesC. elegans (nematode)
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Insert Size (bp)1120
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MutationN/A
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GenBank IDNM_171588
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Entrez Genetwk-46 (a.k.a. CELE_Y39B6A.19)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site Xho1 (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNAOX-twk46 was a gift from Larry Salkoff (Addgene plasmid # 16129 ; http://n2t.net/addgene:16129 ; RRID:Addgene_16129) -
For your References section:
Evolution tunes the excitability of individual neurons. Salkoff L, Butler A, Fawcett G, Kunkel M, McArdle C, Paz-y-Mino G, Nonet M, Walton N, Wang ZW, Yuan A, Wei A. Neuroscience. 2001 . 103(4):853-9. 10.1016/S0306-4522(01)00079-3 PubMed 11301195