pBScMXT-Shaw3
(Plasmid
#16113)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16113 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBScMXT
- Backbone size w/o insert (bp) 3200
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Vector typeXenopus Oocyte expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameVoltage-gated K+ channel
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Alt name5N454
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Alt nameR186.5
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SpeciesC. elegans (nematode)
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Insert Size (bp)1500
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GenBank IDNM_073847
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Entrez Geneshw-3 (a.k.a. CELE_R186.5)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Hind111 (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBScMXT-Shaw3 was a gift from Larry Salkoff (Addgene plasmid # 16113 ; http://n2t.net/addgene:16113 ; RRID:Addgene_16113) -
For your References section:
Eight potassium channel families revealed by the C. elegans genome project. Wei A, Jegla T, Salkoff L. Neuropharmacology. 1996 . 35(7):805-29. 10.1016/0028-3908(96)00126-8 PubMed 8938713
Map uploaded by the depositor.