pcDNAOX-twk7
(Plasmid
#16106)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16106 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNAOX
- Backbone size w/o insert (bp) 5700
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Vector typeMammalian expression & Oocyte expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameVoltage-gated K+ channel
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Alt nameF22B7.7
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Alt name2I598
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Alt nametwk-8
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SpeciesC. elegans (nematode)
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Insert Size (bp)2000
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GenBank IDNM_066502
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Entrez Genetwk-7 (a.k.a. CELE_F22B7.7)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site Xba1 (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNAOX-twk7 was a gift from Larry Salkoff (Addgene plasmid # 16106 ; http://n2t.net/addgene:16106 ; RRID:Addgene_16106) -
For your References section:
Eight potassium channel families revealed by the C. elegans genome project. Wei A, Jegla T, Salkoff L. Neuropharmacology. 1996 . 35(7):805-29. 10.1016/0028-3908(96)00126-8 PubMed 8938713
Map uploaded by the depositor.