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Addgene

T1ACR1_EYFP_pcDNA3.1
(Plasmid #161027)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 161027 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    T1ACR1
  • Species
    Thraustochytrium sp.
  • Insert Size (bp)
    810
  • GenBank ID
    MT002470
  • Tag / Fusion Protein
    • EYFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAGGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    T1ACR1_EYFP_pcDNA3.1 was a gift from John Spudich (Addgene plasmid # 161027 ; http://n2t.net/addgene:161027 ; RRID:Addgene_161027)

  • For your References section:

    RubyACRs, nonalgal anion channelrhodopsins with highly red-shifted absorption. Govorunova EG, Sineshchekov OA, Li H, Wang Y, Brown LS, Spudich JL. Proc Natl Acad Sci U S A. 2020 Sep 15;117(37):22833-22840. doi: 10.1073/pnas.2005981117. Epub 2020 Sep 1. 10.1073/pnas.2005981117 PubMed 32873643
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