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Addgene

pWPXL-p150-MIRΔ
(Plasmid #160995)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160995 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pWPXL
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CHAF1A
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2721
  • Mutation
    MIR (HP1-interacting domain) deletion
  • Entrez Gene
    Chaf1a (a.k.a. AL023013, AL024058, CAF-1, Cac1p)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWPXL-p150-MIRΔ was a gift from Zhiguo Zhang (Addgene plasmid # 160995 ; http://n2t.net/addgene:160995 ; RRID:Addgene_160995)

  • For your References section:

    Chromatin Assembly Factor 1 (CAF-1) facilitates the establishment of facultative heterochromatin during pluripotency exit. Cheng L, Zhang X, Wang Y, Gan H, Xu X, Lv X, Hua X, Que J, Ordog T, Zhang Z. Nucleic Acids Res. 2019 Dec 2;47(21):11114-11131. doi: 10.1093/nar/gkz858. 10.1093/nar/gkz858 PubMed 31586391
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