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pGST-pol1-N2-2A2
(Plasmid #160991)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160991 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGST-parallel
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pol1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    600
  • Mutation
    F58A/D62A
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGST-pol1-N2-2A2 was a gift from Zhiguo Zhang (Addgene plasmid # 160991 ; http://n2t.net/addgene:160991 ; RRID:Addgene_160991)

  • For your References section:

    DNA polymerase alpha interacts with H3-H4 and facilitates the transfer of parental histones to lagging strands. Li Z, Hua X, Serra-Cardona A, Xu X, Gan S, Zhou H, Yang WS, Chen CL, Xu RM, Zhang Z. Sci Adv. 2020 Aug 26;6(35):eabb5820. doi: 10.1126/sciadv.abb5820. eCollection 2020 Aug. 10.1126/sciadv.abb5820 PubMed 32923642
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