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pEGFP-N1 hMOG-beta2-EGFP
(Plasmid #160979)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160979 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Takara Clontech
  • Backbone size w/o insert (bp) 4717
  • Total vector size (bp) 5356
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant beta2
  • Alt name
    MOG beta-2 isoform (according to Swiss Prot)
  • Alt name
    MOG beta-5 isoform (according to UniProt/NCBI)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    639
  • GenBank ID
    NM_206810
  • Entrez Gene
    MOG (a.k.a. BTN6, BTNL11, MOGIG2, NRCLP7)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CMV forward
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-N1 hMOG-beta2-EGFP was a gift from Markus Reindl (Addgene plasmid # 160979 ; http://n2t.net/addgene:160979 ; RRID:Addgene_160979)

  • For your References section:

    Differential Binding of Autoantibodies to MOG Isoforms in Inflammatory Demyelinating Diseases. Schanda K, Peschl P, Lerch M, Seebacher B, Mindorf S, Ritter N, Probst M, Hegen H, Di Pauli F, Wendel EM, Lechner C, Baumann M, Mariotto S, Ferrari S, Saiz A, Farrell M, Leite MIS, Irani SR, Palace J, Lutterotti A, Kumpfel T, Vukusic S, Marignier R, Waters P, Rostasy K, Berger T, Probst C, Hoftberger R, Reindl M. Neurol Neuroimmunol Neuroinflamm. 2021 Jun 15;8(5). pii: 8/5/e1027. doi: 10.1212/NXI.0000000000001027. Print 2021 Jul. 10.1212/NXI.0000000000001027 PubMed 34131067
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