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Addgene

pcDNAOX-IRK3
(Plasmid #16096)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16096 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNAOX
  • Backbone size w/o insert (bp) 5700
  • Vector type
    Mammalian expression & Oocyte expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Inward Rectifying K (potassium) channel family)
  • Alt name
    XO970
  • Alt name
    K04G11.5
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    1720
  • Mutation
    N/A
  • GenBank ID
    NM_077994
  • Entrez Gene
    irk-3

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNAOX-IRK3 was a gift from Larry Salkoff (Addgene plasmid # 16096 ; http://n2t.net/addgene:16096 ; RRID:Addgene_16096)

  • For your References section:

    Eight potassium channel families revealed by the C. elegans genome project. Wei A, Jegla T, Salkoff L. Neuropharmacology. 1996 . 35(7):805-29. 10.1016/0028-3908(96)00126-8 PubMed 8938713
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