Skip to main content
Addgene

plIVMD115
(Plasmid #160937)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160937 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    psh100
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MCP-mCherry-MYC tag
  • Alt name
    MS2 coat protein
  • Species
    S. cerevisiae (budding yeast)
  • Promoter MET25
  • Tag / Fusion Protein
    • MYC tag

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TAGATACAATTCTATTACCCCCATCCATACTCT
  • 3′ sequencing primer GTAAGCGTG ACATAACTAATTACATGACTCGAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    plIVMD115 was a gift from Saeed Tavazoie (Addgene plasmid # 160937 ; http://n2t.net/addgene:160937 ; RRID:Addgene_160937)

  • For your References section:

    In vivo mRNA display enables large-scale proteomics by next generation sequencing. Oikonomou P, Salatino R, Tavazoie S. Proc Natl Acad Sci U S A. 2020 Oct 9. pii: 2002650117. doi: 10.1073/pnas.2002650117. 10.1073/pnas.2002650117 PubMed 33037152
Related items: