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Addgene

plIVMD164
(Plasmid #160936)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160936 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    plIVMD156
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 7700
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MCP-sfGFP-HIS tag, 3'UTR MS2 Stem Loop
  • Alt name
    MS2 coat protein, MS2 hairpin
  • Species
    S. cerevisiae (budding yeast)
  • Promoter MET25
  • Tag / Fusion Protein
    • HIS tag, 3'UTR MS2 Stem Loop

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer TAGATACAATTCTATTACCCCCATCCATACTCT
  • 3′ sequencing primer GTAAGCGTGACATAACTAATTACATGACTCGAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the T247A mutation in sfGFP does not impact plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    plIVMD164 was a gift from Saeed Tavazoie (Addgene plasmid # 160936 ; http://n2t.net/addgene:160936 ; RRID:Addgene_160936)

  • For your References section:

    In vivo mRNA display enables large-scale proteomics by next generation sequencing. Oikonomou P, Salatino R, Tavazoie S. Proc Natl Acad Sci U S A. 2020 Oct 9. pii: 2002650117. doi: 10.1073/pnas.2002650117. 10.1073/pnas.2002650117 PubMed 33037152
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