plIVMD156
(Plasmid
#160934)
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PurposeIn vivo mRNA display Gateway Destination construct
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 160934 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepsh100
- Backbone size w/o insert (bp) 6000
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMCP-Gateway cloning site-HIS tag, 3'UTR MS2 Stem Loop
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Alt nameMS2 coat protein, MS2 hairpin
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)1700
- Promoter MET25
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Tag
/ Fusion Protein
- HIS tag, 3'UTR MS2 Stem Loop (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer TAGATACAATTCTATTACCCCCATCCATACTCT
- 3′ sequencing primer GTAAGCGTGACATAACTAATTACATGACTCGAG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
plIVMD156 was a gift from Saeed Tavazoie (Addgene plasmid # 160934 ; http://n2t.net/addgene:160934 ; RRID:Addgene_160934) -
For your References section:
In vivo mRNA display enables large-scale proteomics by next generation sequencing. Oikonomou P, Salatino R, Tavazoie S. Proc Natl Acad Sci U S A. 2020 Oct 9. pii: 2002650117. doi: 10.1073/pnas.2002650117. 10.1073/pnas.2002650117 PubMed 33037152
