CAG-EnRRbpj
(Plasmid
#160925)
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Purposedominant-negative construct to inhibit Notch signaling
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 160925 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneCAG-EGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEnRRbpj
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SpeciesM. musculus (mouse)
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Mutationfused with an engrailed repressor
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CAG-EnRRbpj was a gift from Mark Emerson (Addgene plasmid # 160925 ; http://n2t.net/addgene:160925 ; RRID:Addgene_160925) -
For your References section:
Notch signaling represses cone photoreceptor formation through the regulation of retinal progenitor cell states. Chen X, Emerson MM. Sci Rep. 2021 Jul 15;11(1):14525. doi: 10.1038/s41598-021-93692-w. 10.1038/s41598-021-93692-w PubMed 34267251