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Addgene

pAGM9121_pDHAS
(Plasmid #160920)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160920 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAGM9121
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dihydroxyacetone synthase promoter
  • Alt name
    DHAS
  • Species
    Hansenula polymorpha
  • Mutation
    BsaI and BbsI domesticated

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAGM9121_pDHAS was a gift from Sylvestre Marillonnet (Addgene plasmid # 160920 ; http://n2t.net/addgene:160920 ; RRID:Addgene_160920)

  • For your References section:

    Improving the Heterologous Production of Fungal Peroxygenases through an Episomal Pichia pastoris Promoter and Signal Peptide Shuffling System. Pullmann P, Weissenborn MJ. ACS Synth Biol. 2021 Jun 2. doi: 10.1021/acssynbio.0c00641. 10.1021/acssynbio.0c00641 PubMed 34075757
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