pT-35
(Plasmid
#16092)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16092 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-KG
- Backbone size w/o insert (bp) 5000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSMT3
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Alt nameYeast SUMO mature form (SMT3gg)
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)294
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Entrez GeneSMT3 (a.k.a. YDR510W)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT-35 was a gift from Yoshiko Kikuchi (Addgene plasmid # 16092 ; http://n2t.net/addgene:16092 ; RRID:Addgene_16092) -
For your References section:
Yeast Ull1/Siz1 is a novel SUMO1/Smt3 ligase for septin components and functions as an adaptor between conjugating enzyme and substrates. Takahashi Y, Kahyo T, Toh-E A, Yasuda H, Kikuchi Y. J Biol Chem. 2001 Dec 28. 276(52):48973-7. 10.1074/jbc.M109295200 PubMed 11577116