pT-61
(Plasmid
#16091)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16091 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET21b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5443
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNFI1
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Alt nameSUMO ligase (E3)
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)2181
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Entrez GeneNFI1 (a.k.a. YOR156C, SIZ2)
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Tags
/ Fusion Proteins
- T7 (N terminal on backbone)
- His (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT-61 was a gift from Yoshiko Kikuchi (Addgene plasmid # 16091 ; http://n2t.net/addgene:16091 ; RRID:Addgene_16091) -
For your References section:
Comparative analysis of yeast PIAS-type SUMO ligases in vivo and in vitro. Takahashi Y, Toh-E A, Kikuchi Y. J Biochem (Tokyo). 2003 Apr . 133(4):415-22. 10.1093/jb/mvg054 PubMed 12761287