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Addgene

At2S3:RUBY
(Plasmid #160906)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160906 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHDE
  • Backbone size w/o insert (bp) 9346
  • Total vector size (bp) 13986
  • Vector type
    Plant Expression
  • Selectable markers
    Spectinomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RUBY and At2S3 promoter
  • Species
    Synthetic; Arabidopsis
  • Insert Size (bp)
    4640
  • Promoter At2S3

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTGTCAAACACTGATAGTTTtaagctggcacaactatatttcc
  • 3′ sequencing primer GCTTACTCAGTTAGGTCTAGCTTATCTTTAATCATATTCCATAGTCCA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    At2S3:RUBY was a gift from Yunde Zhao (Addgene plasmid # 160906 ; http://n2t.net/addgene:160906 ; RRID:Addgene_160906)

  • For your References section:

    A reporter for noninvasively monitoring gene expression and plant transformation. He Y, Zhang T, Sun H, Zhan H, Zhao Y. Hortic Res. 2020 Sep 19;7:152. doi: 10.1038/s41438-020-00390-1. eCollection 2020. 10.1038/s41438-020-00390-1 PubMed 33024566
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